Technology

Recently, it has become apparent that the reliable diagnosis of conditions such as cancer, auto-immune diseases, allergies, and psychological disorders requires simultaneous quantification of a larger set of both DNA and RNA markers. Current methods can only detect these aberrations in separate analysis runs, which increases processing time, effort, and costs. The MODAPLEX multi-modal analysis platform allows the fully automated and simultaneous quantification of up to 2,000 DNA and RNA markers within 3.5 hours.

If you are interested in the Modaplex technology and would like to know more about the advantages, please do not hesitate to contact us.


Modaplex Technology

The automated Modaplex platform overcomes the limitations of conventional methods by combining two traditional biotechnological processes — the polymerase chain reaction (PCR) for DNA amplification and the capillary electrophoresis (CE) separation method performed in parallel. A standard PCR reaction is run with fluorescent-labelled primers. During PCR, amplicons are injected electro-kinetically into the capillary gel. While the PCR reaction continues undisturbed, the injected PCR products migrate through the gel in a size-dependent manner. By combining the size separation and detection after each PCR cycle, real-time data is generated, which enables the quantification of up to 50 targets in one PCR reaction. Complemented by specialized software, Modaplex technology enables the identification and quantification of DNA and RNA targets in a single operation.

 

Multi-Modal Analysis

With the Modaplex technology, users can investigate all types of genetic aberrations using one platform. All Modaplex tests run on the same PCR program. Because of this universal PCR protocol, all tests can be performed simultaneously, which helps in avoiding sample batching. The fact that DNA and RNA targets can be analyzed in one run demonstrates the flexibility of the platform.

 

 

 

Applicable for all life science areas

  • Infectious diseases
  • Oncology
  • Companion diagnostics
  • Food
  • Agriculture
  • Veterinary science
  • Quality control

Compatible with various sample types

  • Plasma, serum, whole blood
  • Liquid biopsies
  • Stool
  • Tissue
  • Formalin-fixed, paraffin-embedded tissue (FFPE)
  • Cell lines/ cell cultures
  • Fungi, bacteria and viruses

Suitable for all biological markers

  • Gene expression
  • Mutations
  • miRNA
  • Copy number variation
  • IncRNA
  • Gene fusion
  • Fragment lenght polymorphism
  • DNA methylation
  • SNP
  • Polysomy
  • Splicing variants

 

Workflow

The Modaplex load-and-walk-away workflow is identical for all tests and combinations of tests. Hands-on activities are reduced to a minimum, limited to the PCR set-up and data analysis. Target amplification, multiple CE-based separations, and target detection are performed automatically, ensuring that the Modaplex run time is always ~ 3 hours.

 

Moda-RA Software

The test systems of Biotype Innovation are used with the multi-modal analysis platform Modaplex. The evaluation is completed with the Moda-RA software (Modaplex Result Analyzer). With this software, the acquired data is imported from the Modaplex device, analyzed, and then summarized in a comprehensive result report.

All detected target genes, controls, and references are subjected to quality control integrated in the Moda-RA software. To do so, a universal color code is used, which immediately notifies the user about the validity and the result interpretation. The software is ready to use and requires no special dongles or decryption procedures.