FGFR Gene Fusion

The Modaplex FGFR Gene Fusion Analysis Kit is a PCR-based multiplex assay for the qualitative detection of gene fusions in FGFR1, FGFR2, and FGFR3. The kit provides all the reagents necessary for the rapid and sensitive detection of eight FGFR gene fusions. Together with the Modaplex technology, the kit provides trustworthy results, while using a workflow that is as simple as a standard PCR.

The Modaplex FGFR Gene Fusion Analysis Kit is intended for research use only purposes and not for diagnostic use.

Product Specifications

TargetsBAG4:FGFR1 (e2:e6)
BAG4:FGFR1 (e1:e8)
FGFR2:CIT (e17:e23)
FGFR3:TACC3 (e17:e4)
FGFR3:TACC3 (e17:e8)
FGFR3:TACC3 (e17:e10)
FGFR3:TACC3 (e17:e11)
FGFR3:TACC3 (e17:e4intra)
Kit ContentFGFR Fusion Primer, FGFR Fusion RT Primer, Taq DNA Polymerase, Buffer,
Positive Control, Nuclease-free Water for NTC and Sample Dilution
Kit Size50 reactions
Sample MaterialTotal human RNA extracted from FFPE tissue
RNA Input12.5ng – 400ng
ReproducibilityTo assess the reproducibility of the FGFR Gene Fusion Analysis Kit, the following tests have been performed:

  • Intra-assay reproducibility
  • Kit and reagent lot-to-lot reproducibility
  • Inter-laboratory reproducibility
  • Inter-Modaplex reproducibility

The Modaplex FGFR Gene Fusion Analysis Kit has passed all tests with a call accuracy of 100%.

TechnologyModaplex
Result InterpretationWith Biotype Innovation Moda-RA software

Workflow

The Modaplex FGFR Gene Fusion Analysis Kit is to be used with the Modaplex technology, a bench-top system that integrates PCR-based amplification with automated capillary electrophoresis (CE)-based detection of amplification products. In combination with the Modaplex FGFR Gene Fusion Test and the analysis software Moda-RA, copy number variations can be assessed in just 3.5 hours.

 

 

A universal PCR amplification profile allows the simultaneous analysis of various biological modalities. Thus, FGFR gene fusion analysis can be combined with any other test for FGFR/FGF genetic alterations in a single run. The workflow and the turnaround time remain unchanged. Therefore, the Modaplex technology is an ideal instrument for multi-analyte assays.

Order Information

Product:Modaplex FGFR Gene Fusion Analysis Kit
Cat. no.:BTI-C001-B1-2-0050

Order Request

 

 

To order the FGFR Gene Fusion Analysis Kit, please e-mail us at order@biotype-innovation.com. Let us know about the specific products you are looking for (incl. their catalog numbers) and we will send you an individual offer customized to your requirements.