Modaplex KRAS Mutation

The Modaplex KRAS Mutation Analysis Kit is a PCR-based multiplex assay that detects and differentiates relevant KRAS mutations.

The mutations of the KRAS gene (Ras oncogene) , which are present in codons 12, 13, 61, and 146, lead to an EGFR receptor-independent activation of the RAS signalling pathway. The KRAS protein (Ras protein) remains permanently active due to its GTPase function and contributes to a stimulation of cell growth. The assay contains all reagents necessary for the rapid and sensitive detection of 13 KRAS mutations.

The Modaplex KRAS Mutation Analysis Kit is intended for research use only and not for diagnostic use.

Product Specification

TargetsA146T, G12A, G12C, G12D, G12R, G12S, G12V, G13C, G13D, Q61HC, Q61HT, Q61L, Q61R
Kit ContentPrimer Mix KRAS, PCR Buffer 6, Modaplex Enhancer 50, Polymerase B, Controls, and Nuclease-Free Water
Kit Size50 rxn
Sample MaterialVerified with artificial sample material spiked into the FFPE (Formalin Fixed Paraffin Embedded) background and pre-characterized DNA samples extracted from FFPE colorectal tissue, if available
DNA Input50 ng
TechnologyModaplex
Result InterpretationWith Biotype’s Excel Meta-Analysis Worksheet

Workflow

The Modaplex KRAS Mutation Analysis Kit is designed for use with the Modaplex platform. This multiplex PCR bench-top system combines qPCR with capillary electrophoresis (CE) in an automated process. All data are processed within 3.5 hours, and the results can be interpreted intuitively using Biotype’s Excel Meta-Analysis Worksheet.

 

Modaplex KNB Workflow

 

A universal PCR amplification profile allows the simultaneous performance of various assay types including fragment analysis, mutation analysis, gene expression, and miRNA.

Thus, KRAS mutation analysis, can be performed together with the analysis of BRAF and NRAS mutations in a single run. For all tests, the Modaplex workflow comprises the same three steps: PCR set up, Modaplex run, and result interpretation.

Order information

Product:Modaplex KRAS Mutation Analysis Kit
Cat. no.:85-10401-0050

Order Request

 

 

To order the KRAS Mutation Analysis Kit, please e-mail us at sales@biotype.de. Let us know about the specific products you are looking for (incl. their catalog numbers), and we will send you an individual offer customized to your requirements.