Modaplex POLE/POLD1

The Modaplex POLE/POLD1 Mutation Analysis Kit is a PCR-based multiplex assay that detects and differentiates relevant exonuclease domain mutations (EDMs) in the polymerase epsilon and polymerase delta-1 subunits. The kit contains all reagents necessary for the rapid and sensitive detection of 17 POLE EDMs and 3 POLD1 EDMs.

In combination with MSI status, information on POLE/POLD1 mutations could provide valuable therapy decisions for patients with MSS phenotypes in colorectal and endometrial tumors.

The Modaplex POLE/POLD1 Mutation Analysis Kit is intended for research use only and not for diagnostic use.

Product Specification


POLE: T278M, P286S, P286L, P286H, P286R, S297A, S297F, F367S, V411L (G>C), V411L (G>T), H422N, L424V, P436R, M444K, A456P, S459F, A465VPOLD1: D316N, C319Y, S478N


POLD1: D316N, C319Y, S478N

Kit ContentPrimer Mix, Modaplex Buffer 3, Modaplex Enhancer 50, Polymerase T, Controls, and Nuclease-Free Water
Kit Size50 rxn
Sample Material

Verified with:  artificial sample material spiked into the FFPE (Formalin Fixed Paraffin Embedded) background

Tested with:    FFPE Endometrial and Colorectal Cancer

DNA Input

Input range:  2 – 8ng

Optimum:    4ng

Result InterpretationWith Biotype’s Moda-RA software


The Modaplex POLE/POLD1 Mutation Analysis Kit is designed for use with the Modaplex platform. This multiplex PCR bench-top system combines qPCR with capillary electrophoresis (CE) in an automated process. All data is processed in just 3.5 hours, and the results can be interpreted intuitively using Biotype’s Modaplex Result Analyzer (Moda-RA) software.



A universal PCR amplification profile allows for the simultaneous performance of various assay types such as fragment analysis, mutational analysis, gene expression, miRNA, among many others. For all tests, the Modaplex workflow comprises the same three steps: PCR set up, Modaplex run and result interpretation.

Order information

Product:Modaplex POLE/POLD1 Mutation Analysis Kit
Cat. no.:BTI-C003-C1-2-0050

Order Request



To order the POLE/POLD1 Mutation Analysis Kit, please e-mail us at Let us know about the specific products you are looking for (incl. their catalog numbers), and we will send you an individual offer customized to your requirements.