MSI

The Modaplex MSI Analysis Kit is a PCR-based multiplex assay for the qualitative assessment of microsatellite instability. With a set of eight microsatellite markers (Bat-25, Bat-26, NR-21, NR-24, Mono27, D2S123, D5S346, D17S250), the kit detects instability by comparing microsatellite profiles from cancerous and normal matching tissue. The set of eight markers allows for the assessment of MSI-High status based on established and standardized guidelines known as the Bethesda and the revised Bethesda guidelines.

The Modaplex MSI Analysis Kit is intended for research use only purposes and not for diagnostic use.

Product Specification

TargetsBat-25, Bat-26, NR-21, NR-24, Mono27, D2S123, D5S346, D17S250
Kit ContentPrimer Mix, Modaplex Buffer, Modaplex Enhancer, Polymerase T, Controls and Nuclease-free Water
Kit Size50 rxn
Sample Material

Verified with: FFPE – Formalin Fixed Paraffin Embedded – Colorectal Cancer

Tested with:   FFPE Endometrial Cancer

DNA Input

Input range:   0.5 – 5ng

Optimum:      2ng

TechnologyModaplex
Result InterpretationWith Biotype Innovation’s Moda-RA software

Workflow

The Modaplex MSI Analysis Kit is designed for use with the Modaplex platform. This multiplex PCR bench-top system combines qPCR with capillary electrophoresis (CE) in an automated process. All data is processed in just 4 hours, and the results can be interpreted intuitively using Biotype Innovation’s Modaplex Result Analyzer (Moda-RA) software.

 

 

A universal PCR amplification profile allows for the simultaneous performance of various assay types such as fragment analysis, mutational analysis, gene expression, miRNA, among many others. Thus, MSI fragment analysis and polymerase epsilon/ polymerase delta 1 mutation detection can be performed in a single run. For all tests, the Modaplex workflow comprises the same three steps, PCR set up, Modaplex run and result interpretation, which are completed in less than 5 hours.

Order information

Product:Modaplex MSI Analysis Kit
Cat. no.:BTI-C002-E1-2-0050

Order Request

 

 

To order the MSI Analysis Kit, please e-mail us at order@biotype-innovation.com. Let us know about the specific products you are looking for (incl. their catalog numbers), and we will send you an individual offer customized to your requirements.